Context
This case study investigates structural plasticity signals in hippocampal tissue and tracks morphology linked to tenascin-C pathways.
Research note
Analyzing Synaptic Structures in Hippocampal Neurons
Raw image and processed view
A simple comparison of microscopy signal and processed segmentation output.
Annotated image
Highlighted points connect image regions to interpretation notes.
Elevated synaptic clustering in CA1 suggests active local remodeling.
This hotspot aligns with stronger marker colocalization after processing.
Dense perineuronal signal region with stable morphology across samples.
Closer view
A closer look at the processed microscopy image.
Process
1. Acquisition
Confocal imaging of hippocampal sections with consistent exposure parameters.
2. Segmentation
ImageJ and IMARIS pipelines to isolate structures and control for noise.
3. Quantification
Feature extraction, density metrics, and cross-group statistical comparison in Python.
4. Visualization
Annotated overlays and interpretive figure design for publication-ready communication.